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Tetsushi Sakuma
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Journal Articles

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Journal: Biology Open
Biol Open (2017) 6 (5): 706–713.
https://doi.org/10.1242/bio.025122
Published: 10 April 2017
View article titled, Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice
Open the PDF for Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice in another window
Includes: Supplementary data
Journal Articles

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Noriyuki Yanaka, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Journal: Biology Open
Biol Open (2016) 5 (8): 1142–1148.
https://doi.org/10.1242/bio.019349
Published: 7 July 2016
View article titled, Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice
Open the PDF for Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice in another window
Includes: Supplementary data
Journal Articles

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

Ken-ichi T. Suzuki, Yukiko Isoyama, Keiko Kashiwagi, Tetsushi Sakuma, Hiroshi Ochiai, Naoaki Sakamoto, Nobuaki Furuno, Akihiko Kashiwagi, Takashi Yamamoto
Journal: Biology Open
Biol Open (2013) 2 (5): 448–452.
https://doi.org/10.1242/bio.20133855
Published: 4 March 2013
View article titled, High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
Open the PDF for High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos in another window
Includes: Supplementary data
Journal Articles

Quantitative assay for TALEN activity at endogenous genomic loci

Yu Hisano, Satoshi Ota, Kazuharu Arakawa, Michiko Muraki, Nobuaki Kono, Kazuki Oshita, Tetsushi Sakuma, Masaru Tomita, Takashi Yamamoto, Yasushi Okada, Atsuo Kawahara
Journal: Biology Open
Biol Open (2013) 2 (4): 363–367.
https://doi.org/10.1242/bio.20133871
Published: 12 February 2013
View article titled, Quantitative assay for TALEN activity at endogenous genomic loci
Open the PDF for Quantitative assay for TALEN activity at endogenous genomic loci in another window
Includes: Supplementary data