First data on microflora of loggerhead sea turtle (Caretta caretta) nests from the coastlines of Sicily
Summary: We report for the first time the microflora of six loggerhead sea turtle nests on the Sicilian coast, and associated failed hatchings.
Leucine-rich repeat containing 8A contributes to the expansion of brain ventricles in zebrafish embryos
Summary: This study provides evidence to demonstrate that organic osmolytes such as taurine contribute to the inflation of the brain ventricle via the volume-sensitive organic osmolyte-anion channel during zebrafish embryogenesis.
An insertion mutation of ERBB2 enhances breast cancer cell growth and confers resistance to lapatinib through AKT signaling pathway
Summary: We report a novel insertion mutation of ERBB2, which enhances breast cancer cell growth and confers resistance to lapatinib. Combination of the AKT inhibitor with lapatinib can overcome this resistance.
Summary: In this study, bird-borne cameras were used to investigate the factors influencing capture success, chase and handling time, and prey profitability in Australasian gannets.
BAG6 contributes to glucose uptake by supporting the cell surface translocation of the glucose transporter GLUT4
Summary: BAG6 is critical for the insulin-stimulated translocation of GLUT4 from its peri-nuclear storage compartments to the cell surface.
New approach and new permutation tests with R programs for analyses of false-negative-contaminated data in medicine and biology
Summary: This article presents a new R-script for analysis of data containing a subpopulation of false-negative individuals, for example, the population of infected individuals that scored negatively in a serological assay.
Selective targeting of mRNA and the following protein synthesis of CaMKIIα at the long-term potentiation-induced site
Summary: Selective targeting of mRNA and the following protein synthesis of CaMKIIalpha at neuronal plasticity-induced sites may be one of important processes for establishment of long-term memory.
Assessing the role of historical temperature regime and algal symbionts on the heat tolerance of coral juveniles
Summary: Survival of coral juveniles under heat stress can be significantly improved by breeding corals from historically warmer reefs with those from cooler reefs, and the provisioning with heat tolerant symbionts.
The sequential phosphorylation of PHF10 subunit of the PBAF chromatin-remodeling complex determines different properties of the PHF10 isoforms
Summary: The sequential phosphorylation of the linker domain of PHF10 subunit of PBAF chromatin remodeling complex is triggered by two primarily phosphorylated serines and determines the different properties of PHF10 isoforms.
Forcing contacts between mitochondria and the endoplasmic reticulum extends lifespan in a Drosophila model of Alzheimer's disease
Summary: Enhancing mitochondria–ER contacts ameliorates locomotor phenotypes and extends lifespan in a fly model of Alzheimer's disease.
Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants
Summary: We analysed a cellular structure formed by changes associated with exposure to light in the compound eyes of Drosophila white mutants, white; yata mutants and aged flies.
Short-term cyclical stretch phosphorylates p38 and ERK1/2 MAPKs in cultured fibroblasts from the hearts of rainbow trout, Oncorhynchus mykiss
Summary: Here we demonstrate that 10% deformation at 0.33 Hz of trout cardiac fibroblasts stimulates p38-JNK-ERK mitogen activated protein kinase pathways. This suggests that mechanotransductive pathways trigger cardiac remodeling in trout.
Establishment of a novel culture method for maintaining intestinal stem cells derived from human induced pluripotent stem cells
Summary: This study describes a novel two-dimensional culture method for maintaining intestinal stem cells (ISCs) derived from human iPS cells. The enterocytes differentiated from maintained ISCs showed pharmacokinetics functions.
METHODS & TECHNIQUES
Defined extracellular ionic solutions to study and manipulate the cellular resting membrane potential
Summary: We describe a simplified roadmap to study the role of bioelectricity, in particular individual ions, in cell biological processes.
Summary: An improved in-gel activity assay visualized respiratory chain complexes III, IV and supercomplexes through cytochrome c. Pre-incubation of detergents enhanced the in-gel activity assay.