Whole-transcriptome splicing profiling of E7.5 mouse primary germ layers reveals frequent alternative promoter usage during mouse early embryogenesis
Summary: This study seeks to capture the alternative splicing landscape during mouse gastrulation, underlining the potential importance of alternative promoter usage in mammalian early embryogenesis.
Summary: Mediator 12, a transcriptional coactivator, greatly enhances Wnt signaling in the developing embryo. Separate from its role in Wnt signaling, Mediator 12 is required for oxytocin expression.
Inhibition of miR-34a-5p alleviates hypoxia-reoxygenation injury by enhancing autophagy in steatotic hepatocytes
Summary: Our study establishes an in vitro model for studying H/R injury in fatty liver, and shows that MiR-34a plays an important role in worsening H/R injury, likely by suppressing autophagy in steatotic hepatocytes.
Summary: Trypanosomes do not have a spindle checkpoint but have an ability to regulate the timing of nuclear division by modulating the cyclin B protein level.
Summary: We report, for the first time, a metabolic map of abalone metabolism in response to functional hypoxia, compiled from results obtained by metabolomics analysis.
Summary: Teneurins are transmembrane proteins found in the developing nervous system that are related to bacterial toxins. Teneurins also have cytotoxic properties that may help regulate apoptosis or pruning.
Summary: Oyster sperm motility is not triggered in an acidic environment and motility is controlled by internal pH alkalinization, mediated by a Na+/H+ exchanger.
Summary: ZFP36L2 is a cell cycle-regulated RNA-binding protein, the abundance of which is regulated post-translationally. This protein is especially accumulated in and critical for the survival of DNA-damaged cells.
Summary: The development of alginate:collagen composite hydrogel microspheres of adjustable size and degradation speed is described as a new platform for delivery of pro-angiogenic FGF-2 or pro-angiogenic cells.
Summary: We have developed a novel reporter line, free from complicating factors associated with previous alleles, for monitoring Dll4 expression, at a cellular resolution, in the developing and adult mouse.
METHODS & TECHNIQUES
Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation
Summary: A streamlined and adaptable cell imaging method for quantitative analysis of chromatin organisation in mammalian cells, and its application, reveal a new role for ATM in regulating chromatin structure.
Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout, Oncorhynchus mykiss
Summary: Understanding chemical uptake from the diet is difficult in live fish: we developed long-term intestinal cell cultures that enables the science and provides an alternative method.