Vertically- and horizontally-transmitted memories – the fading boundaries between regeneration and inheritance in planaria
Summary: In this hypothesis paper we re-evaluate canonical ideas about the interaction between developmental, genetic and evolutionary processes through the lens of planaria, an invertebrate model organism which challenges fundamental assumptions regarding reproduction.
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation
Summary: We describe the role of FAK and focal adhesion proteins in capacitation, acrosome reaction, polymerization and remodeling of actin cytoskeleton, and how inhibition of FAK affects sperm physiology.
Modulation of digestive physiology and biochemistry in Mytilus californianus in response to feeding level acclimation and microhabitat
Summary: We show for the first time that the mussel Mytilus californianus has a flexible digestive strategy to deal with varying environmental conditions that are based upon their position on the shore.
How great white sharks nourish their embryos to a large size: evidence of lipid histotrophy in lamnoid shark reproduction
Summary: Great white sharks (Carcharodon carcharias) nourish their embryos with a lipid-rich ‘milk’ secreted by uterine epithelium during the early gestation period, before the onset of oophagy.
Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination
Summary: Twist1+ epithelium retains intercellular junctions and proliferative capacity. During dissemination, Twist1+ cells retain adhesive capacity and exhibit features of both amoeboid and mesenchymal migration modes.
Summary: Detailed analysis of fictive motor patterns unveils endogenous characteristics of the cockroach thoracic locomotion control networks and their interrelations and enables an explanatory connectivity model.
Allometric growth and development of organs in ballan wrasse (Labrus bergylta Ascanius, 1767) larvae in relation to different live prey diets and growth rates
Summary: Allometric growth patterns of ballan wrasse were not affected by different diets, and larval functional development was found to be dependent on size and not age or growth rate.
Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging
Summary: We applied matrix-assisted laser desorption/ionization−mass spectrometry imaging analyses to identify and visualize metabolomic molecular markers in tadpoles of Xenopus tropicalis. We found new molecular markers in various tissues and cells.
miR-20a regulates proliferation, differentiation and apoptosis in P19 cell model of cardiac differentiation by targeting Smoothened
Summary: Our findings may bring a new viewpoint into the mechanisms of cardiac abnormalities and suggest that miR-20a may be a potential new therapeutic target for congenital heart diseases.
Summary: Insulator speckles have been discussed to be either storage places for insulator proteins or to represent sites of long-range chromatin interaction. Here we demonstrate their association with interacting chromatin.
Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages
Summary: We identified cells co-expressing differentiation markers of Sertoli and peritubular myoid cell lineages in X. tropicalis through the establishment and characterization of cell culture derived from juvenile testis.
RPGR, a prenylated retinal ciliopathy protein, is targeted to cilia in a prenylation- and PDE6D-dependent manner
Summary: RPGR is a ciliary protein that functions as a scaffold to recruit cargo-loaded PDE6D to cilia. Our study shows that RPGR is also a cargo of PDE6D.
Summary: Although mutations in individual microtubule glutamylating enzymes do not disrupt essential microtubule functions in C. elegans, combining mutations in three enzymes uncovers a redundant function for glutamylation in male mating.
Summary:35S-labeled sulfate incorporated into various cells of coral demonstrates that photosynthesis of endosymbiotic algae contributes to the synthesis and utilization of sulfur compounds.
Summary: We examined the genomes of Drosophila-associated bacteria to identify factors that allow survival within the host. These preliminary studies may point at bacterial products that influence host health.
Summary: A symbiotic alga Chlorococcum sp. GD was successfully isolated from the soil moss and successfully cultivated under axenic conditions. It has nearly 40% lipid content, and thus appears to have potential for use in biodiesel production.
METHODS & TECHNIQUES
Rabbit Achilles tendon full transection model – wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery
Summary: The rabbit Achilles tendon full transection model can be used to study adhesion extent in a controlled way. It also mimics the biomechanics of human hand flexor tendons.
3D for the people: multi-camera motion capture in the field with consumer-grade cameras and open source software
Summary:Argus is a free and open source toolset for using consumer grade cameras to acquire 3D kinematic data in field settings.
Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters
Summary: Commonly used fixation protocols during immunolabelling can result in artefactual protein distribution. We highlight the artefacts in images and provide fixation conditions for studying membrane receptor organisation.