No discrimination shock avoidance with sequential presentation of stimuli but shore crabs still reduce shock exposure
Summary: There was no discrimination shock avoidance with sequential presentation of stimuli but, consistent with pain, shore crabs used other tactics to reduce shock exposure.
Summary: We identify IER3 mRNA as a newly discovered MCPIP1 target using recently described IF-based procedures, and also identify a conserved element involved in MCPIP1-dependent IER3 transcript destabilization.
Combined cisplatin and aurora inhibitor treatment increase neuroblastoma cell death but surviving cells overproduce BDNF
Summary: Cisplatin increases endogenous BDNF in MYCN-expanded neuroblastoma cells. Additional treatment with aurora kinase inhibitor PHA-680632 increases cell death but surviving cells overproduce BDNF, mainly by increased translation of exon 6.
Metabolite profile of a mouse model of Charcot–Marie–Tooth type 2D neuropathy: implications for disease mechanisms and interventions
Summary: A metabolomics analysis of a mouse model of Charcot–Marie–Tooth type 2D neuropathy revealed a clear distinction between mutant and control samples, and the therapeutic potential of a subset of these changes was explored.
Summary: Australasian gannets from two colonies in south-eastern Australia were equipped with GPS and miniature video data loggers to record foraging location and the prey preference.
Summary: Early emerging problems during oogenesis, such as DNA double-strand breaks, can affect chromosome duplication and segregation in embryogenesis in Drosophila. Moreover, environmental cues including temperature are important for proper oogenesis.
A genetic link between epigenetic repressor AS1-AS2 and a putative small subunit processome in leaf polarity establishment of Arabidopsis
Summary: This paper reports the importance of cooperative action between the nucleus-localized epigenetic repressor and the nucleolus-localized proteins involved in ribosomal RNA processing for polarity establishment of Arabidopsis leaves.
Metabolic cost of osmoregulation in a hypertonic environment in the invasive African clawed frog Xenopus laevis
Summary: A significant increase in energy expenditure of Xenopus laevis exposed to hyperosmotic conditions is associated with urea concentration and a long-term effect of increasing the metabolic enzyme activities of liver and heart.
Summary: Ca2+ induces a monomer-dimer switch of MICU2, with the associated conformational change governed by its first EF-hand, but not influenced by the C-helix domain. A disulfide bond and salt bridge each contribute to MICU1-MICU2 heterodimer formation.
Differences in cardiovascular toxicities associated with cigarette smoking and snuff use revealed using novel zebrafish models
Summary: We analyze common and distinct developmental and cardiovascular toxicities caused by cigarette smoke and snuff, and include a new zebrafish embryo model for studying abdominal aortic aneurysm.
A lipidomics study reveals hepatic lipid signatures associating with deficiency of the LDL receptor in a rat model
Summary: An LDL receptor knockout rat model was generated which offers a new hypercholesterolemia model. A lipidomics analysis reveals hepatic lipid signatures associating with LDLR deficiency in rats.
METHODS & TECHNIQUES
Summary: We report the generation and characterization of set of monoclonal and polyclonal antibodies for labeling the major compartments of the secretory and endocytic pathways in Drosophila melanogaster.
Laser-based technique for controlled damage of mesenchymal cell spheroids: a first step in studying reparation in vitro
Summary: The technique of precise nanosecond laser microsurgery of mesenchymal cell spheroids was used to develop a new simple reproducible model for studying repair and regeneration in vitro.
PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins
Summary: We show that proper PFA fixation allows high-quality super-resolution imaging of the actin cytoskeleton and can outperform gold-standard glutaraldehyde fixation for imaging of actin-binding proteins.