Patient-derived organoids grown in three-dimensional cultures provide an excellent platform for phenotypic high-throughput screening and drug response research. Organoid technology has been applied to study stem cell biology and various human pathologies. This study investigates the characteristics and cellular morphology of organoids derived from primary human nasal epithelial cells (HNECs) of chronic rhinosinusitis (CRS) patients. Nasal organoids were cultured up to 20 days and morphological, cell composition, and functional parameters were measured by immunofluorescence, RT-qPCR, western-blot and FACS analysis. The results showed that nasal organoids expressed the stem cell marker Leucine Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5), and markers for apical junction genes, goblet cells and ciliated cells. Moreover, we were able to regrow and expand the nasal organoids well after freezing and thawing. This study promise an effective and feasible method for development of human nasal organoids, suitable for the phenotypic high-throughput screening and drug response research.

This content is only available via PDF.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Article PDF first page preview

Article PDF first page preview