Patient-derived organoids grown in three-dimensional cultures provide an excellent platform for phenotypic high-throughput screening and drug response research. Organoid technology has been applied to study stem cell biology and various human pathologies. This study investigates the characteristics and cellular morphology of organoids derived from primary human nasal epithelial cells (HNECs) of chronic rhinosinusitis (CRS) patients. Nasal organoids were cultured up to 20 days and morphological, cell composition, and functional parameters were measured by immunofluorescence, RT-qPCR, western-blot and FACS analysis. The results showed that nasal organoids expressed the stem cell marker Leucine Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5), and markers for apical junction genes, goblet cells and ciliated cells. Moreover, we were able to regrow and expand the nasal organoids well after freezing and thawing. This study promise an effective and feasible method for development of human nasal organoids, suitable for the phenotypic high-throughput screening and drug response research.
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RESEARCH ARTICLE|
22 April 2022
Characterization of human nasal organoids from chronic rhinosinusitis patients
Ramezanpour Mahnaz,
Ramezanpour Mahnaz
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Bolt Harrison,
Bolt Harrison
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
2
College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide, South Australia, 5001
, Australia
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Karen Hon,
Karen Hon
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Shaghayegh Gohar,
Shaghayegh Gohar
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Rastin Hadi,
Rastin Hadi
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
3
School of Chemical Engineering, The University of Adelaide, South Australia 5005
, Australia
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Fenix Kevin Aaron,
Fenix Kevin Aaron
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Psaltis Alkis James,
Psaltis Alkis James
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Wormald Peter-John,
Wormald Peter-John
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
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Vreugde Sarah
Vreugde Sarah
*
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
*Author for correspondence: sarah.vreugde@adelaide.edu.au
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Ramezanpour Mahnaz
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Bolt Harrison
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
2
College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide, South Australia, 5001
, Australia
Karen Hon
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Shaghayegh Gohar
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Rastin Hadi
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
3
School of Chemical Engineering, The University of Adelaide, South Australia 5005
, Australia
Fenix Kevin Aaron
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Psaltis Alkis James
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Wormald Peter-John
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
Vreugde Sarah
*
1
Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network (Basil Hetzel Institute), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide
, Australia
*Author for correspondence: sarah.vreugde@adelaide.edu.au
Received:
06 Feb 2022
Accepted:
06 Apr 2022
© 2022. Published by The Company of Biologists Ltd
2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Biol Open bio.059267.
Article history
Received:
06 Feb 2022
Accepted:
06 Apr 2022
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Citation
Ramezanpour Mahnaz, Bolt Harrison, Karen Hon, Shaghayegh Gohar, Rastin Hadi, Fenix Kevin Aaron, Psaltis Alkis James, Wormald Peter-John, Vreugde Sarah; Characterization of human nasal organoids from chronic rhinosinusitis patients. Biol Open 2022; bio.059267. doi: https://doi.org/10.1242/bio.059267
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